Simultaneous measurement of photosynthesis and chlorophyll fluorescence

Chlorophyll Fluorescence Module (CFM)

The CIRAS-2, with optional integrated Chlorophyll Fluorescence Module (CFM), is a powerful tool for photosynthesis research. The CFM has been developed in cooperation with Hansatech Instruments for complete integration with the CIRAS-2 Portable Photosynthesis System.

The CFM provides measurement of chlorophyll fluorescence using the pulse amplitude modulation (PAM) technique. The pulsed fluorescence beam is used to probe the efficiency of light-use for photosystem ll photochemistry.

Chlorophyll Fluorescence Module

Our modified LED light unit (as shown to the left) includes full chlorophyll fluorescence detection capabilities while retaining all normal functionality as an actinic light source for gas exchange measurements. The modified unit provides the modulation beam, saturating pulses, far-red illumination and fluorescence detection required for chlorophyll fluorescence measurement in a remarkably compact, solid-state and lightweight unit. It does not require additional power sources for operation. The following chlorophyll fluorescence parameters are measured and calculated on demand by the CIRAS-2:

Fo, Fm, Fv, Fv/Fm (Max Yield), Fs, Fm', Fo', fPSII or ∆F/Fm' (Yield), qP, qNP, NPQ, PAR, TEMP and ETR.

CFM Technical Specification
Modulating Beam	627 nm (Red)
Actinic Light	Red and white LEDs Range: 0-2,000 µmol m^-2 s^-1
Saturation Light	Red and white LED's Range: 0-6,000 µmol m^-2 s^-1
Far Red Light	Two 735 nm LED's
Detector	PIN photodiode with >700 nm filter
Detector Method	Rapid pulse peak tracking
Leaf Area	2.5 cm²

CIRAS-2 Remote Control Software

Remote Control Software

The system is also supplied with software allowing remote control operation of the CIRAS-2 with integral Chlorophyll Fluorescence Module (CFM) from a PC. Powerful, flexible software allows for simple, individual measurements to more complex, automated and pre-programmable measurements.

Chlorophyll Fluorescence Adaptation

Many of our leaf cuvettes can be adapted to work with other available fluorometers for simultaneous measurement of leaf gas exchange and chlorophyll fluorescence.

For more information on this, please contact PP Systems.