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Chlorophyll
Fluorescence Module-CFM
Developed in cooperation with
Hansatech Instruments, Ltd. (UK), the CFM has been
developed for complete integration with the CIRAS range
of systems. |
Simultaneous leaf gas exchange and
chlorophyll fluorescence in one truly integrated and
portable system
The CFM provides measurement of chlorophyll fluorescence
using the Pulse Amplitude Modulation (PAM) technique.
The pulsed fluorescence that is induced by the
modulation beam is used to probe the efficiency of
light-use for photosystem II photochemistry. Our
LED light unit has been extensively redeveloped to
include chlorophyll fluorescence detection capabilities,
while retaining all normal functionality as an actinic
light source for gas exchange measurements. The modified
unit provides the modulating beam, saturating pulses,
far-red illumination and fluorescence detection required
for fluorescence measurement, in a remarkably compact,
solid-state and lightweight unit. This requires no
additional power sources.
When part of a CIRAS-2 system, the following gas
exchange and chlorophyll fluorescence parameters are
measured and recorded:
| Leaf Gas
Exchange |
Assimilation (Pn), Internal
CO2 (Ci), Stomatal Conductance (Gs),
Evaporation/Transpiration (E), Vapour Pressure
Deficit (VPD) |
| Chlorophyll
Fluorescence |
Fo, Fm, Fv, Fv/Fm (Max
Yield), Fs, Fm', Fo', ΦPSII or ΔF/Fm' (Yield),
qP, qNP, NPQ, PAR, TEMP and ETR |
CIRAS-2 Data
Sheet
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Chlorophyll
Fluorescence Adaption
Our PLC5 (B) Automatic Broad Leaf
Cuvette shown with quartz halogen light unit and special
fiber optic for use with Hansatech FMS1
|
Several of our leaf cuvettes can be adapted for use with
commercially available fluorometers. In
particular, we have developed a simple yet powerful
adaptation to the Hansatech FMS1 and FMS2 pulse
modulated fluorometers. A special fiber optic
cable with extended tip (8mm diameter) can be used with
many of our light units. It enables the fiber tip
to get close to the cuvette window without intruding on
the leaf illumination. If ordering a chlorophyll
fluorescence adaption for use with Walz fluorometers,
please provide us with the diameter and length of the
fiber tip.
Contact PP
Systems for more details. |
